Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 1 | MIT 7.01SC Fundamentals of Biology
Agarose Gel Electrophoresis, DNA Sequencing, PCR, Lecture Video Excerpt 1 Instructor: Eric Lander View the complete course: http://ocw.mit.edu/7-01SCF11 ...
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So far, there are a lot of ways to clone genes. I've given you two. Later in the course, I'll give you a third, but we cloned it here by its function, complementation. We cloned it here on the basis of the protein it actually made. Now, how do we analyze these genes? How do you know which gene we're dealing with? How do we study it? Let's turn to that. Analyzing our gene. Well, let's suppose we have our cell, maybe it's our yeast cell. It's Arg1 again. We'll go back to yeast. It's got its yeast DNA it's also got this circle that has our plasmid here. Circle is plasmid. How are we going to study that gene? We know that yeast is able to now grow. We can grow up that yeast. We can purify DNA from it. But wait a second. How do we get our pl...
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So far, there are a lot of ways to clone genes. I've given you two. Later in the course, I'll give you a third, but we cloned it here by its function, complementation. We cloned it here on the basis of the protein it actually made. Now, how do we analyze these genes? How do you know which gene we're dealing with? How do we study it? Let's turn to that. Analyzing our gene. Well, let's suppose we have our cell, maybe it's our yeast cell. It's Arg1 again. We'll go back to yeast. It's got its yeast DNA it's also got this circle that has our plasmid here. Circle is plasmid. How are we going to study that gene? We know that yeast is able to now grow. We can grow up that yeast. We can purify DNA from it. But wait a second. How do we get our pl...
