Basic Mechanisms of Cloning, excerpt 1 | MIT 7.01SC Fundamentals of Biology
Basic Mechanisms of Cloning, excerpt 1 Instructor: Eric Lander View the complete course: http://ocw.mit.edu/7-01SCF11 License: Creative Commons BY-NC-SA ...
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PROFESSOR: So, first step, we need to cut our DNA. Step one, cut, which is going to be DNA restriction enzymes. It turns out, quite remarkably, that if I have a sequence of DNA, five prime A G C T A G A A T T C T T A C C three prime, and we'll come backwards filling in the sequence. It turns out that molecular biologists are so cool that they invented a protein that is able to recognize that six letter sequence, G-A-A-T-T-C. And it is able-- actually it's G-A-A-T-T-C on this strand, but what is it coming back? It's G-A-A-T-T-C. It's the same thing. So actually this is a palindrome. That's kind of nice. It's a palindrome-- it's a reverse palindrome. It's the same word spelled backwards on the other strand. So what it does is it cuts it like that. ...
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PROFESSOR: So, first step, we need to cut our DNA. Step one, cut, which is going to be DNA restriction enzymes. It turns out, quite remarkably, that if I have a sequence of DNA, five prime A G C T A G A A T T C T T A C C three prime, and we'll come backwards filling in the sequence. It turns out that molecular biologists are so cool that they invented a protein that is able to recognize that six letter sequence, G-A-A-T-T-C. And it is able-- actually it's G-A-A-T-T-C on this strand, but what is it coming back? It's G-A-A-T-T-C. It's the same thing. So actually this is a palindrome. That's kind of nice. It's a palindrome-- it's a reverse palindrome. It's the same word spelled backwards on the other strand. So what it does is it cuts it like that. ...
