Basic Mechanisms of Cloning, excerpt 2 | MIT 7.01SC Fundamentals of Biology
Basic Mechanisms of Cloning, excerpt 2 Instructor: Eric Lander View the complete course: http://ocw.mit.edu/7-01SCF11 License: Creative Commons BY-NC-SA ...
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PROFESSOR: Now having cut DNA, we then need to do something else. What's the next thing we have to do? AUDIENCE: Paste it. PROFESSOR: Paste it right. Oh, I put past. It should be paste. There we go. We should paste it. So, how are we going to paste our DNA? So let's take some human DNA. We'll take your human DNA. We'll add EcoR1 to it. And your human DNA is going to get cut up in lots of little pieces of length about 4,000. And I'm writing R1 at the ends because these pieces of DNA have EcoR1 sites. Now, I can take that human DNA and I can combine it with other pieces of DNA. Here's a piece of DNA. Remember it had this overhang like that. T-T-A-A was this piece here of human DNA. And I could take another piece of DNA that matched it a A-A-T-T. ...
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PROFESSOR: Now having cut DNA, we then need to do something else. What's the next thing we have to do? AUDIENCE: Paste it. PROFESSOR: Paste it right. Oh, I put past. It should be paste. There we go. We should paste it. So, how are we going to paste our DNA? So let's take some human DNA. We'll take your human DNA. We'll add EcoR1 to it. And your human DNA is going to get cut up in lots of little pieces of length about 4,000. And I'm writing R1 at the ends because these pieces of DNA have EcoR1 sites. Now, I can take that human DNA and I can combine it with other pieces of DNA. Here's a piece of DNA. Remember it had this overhang like that. T-T-A-A was this piece here of human DNA. And I could take another piece of DNA that matched it a A-A-T-T. ...
