Constructing and Screening a Recombinant DNA Library | MIT 7.01SC Fundamentals of Biology
Constructing and Screening a Recombinant DNA Library Instructor: Eric Lander View the complete course: http://ocw.mit.edu/7-01SCF11 License: Creative ...
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PROFESSOR: Good morning, good morning. So last time, we talked about the most remarkable biochemical purification procedure ever invented-- cloning. You remember the issue with biochemistry. You're going to grind up a cell, you're going to take the contents and run it over different kinds of separation columns or centrifuge it or things, in order to separate some things from other things. The problem with purifying a gene that way, away from all the rest of DNA in a cell, is that the gene has exactly the same biochemical properties as all the other genes. How in the world are you going to do it? The solution was devilishly simple and devilishly complex at the same time. All you have to do to purify something is dilute it. If I take any substance an I add enough water, it'...
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PROFESSOR: Good morning, good morning. So last time, we talked about the most remarkable biochemical purification procedure ever invented-- cloning. You remember the issue with biochemistry. You're going to grind up a cell, you're going to take the contents and run it over different kinds of separation columns or centrifuge it or things, in order to separate some things from other things. The problem with purifying a gene that way, away from all the rest of DNA in a cell, is that the gene has exactly the same biochemical properties as all the other genes. How in the world are you going to do it? The solution was devilishly simple and devilishly complex at the same time. All you have to do to purify something is dilute it. If I take any substance an I add enough water, it'...
